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德国Vivascience 蛋白纯化试剂盒
The
separation of pure proteins from complex mixtures is a key process in
biomedical research and other biological disciplines. Vivascience is
offering Vivapure spin columns based on an innovative and powerful
membrane adsorber technology for the purification of proteins. The porous
membrane adsorber matrix enables fast, reproducible and scalable protein
purification.
The
Vivapure advantage:
·
Fast
- Cost
effective
- Centrifuge
based
- Easy
to use
- Parallel
processing
- Reproducible
·
Available in a range of different membrane chemistries
(e.g. range of strong or weak ion exchangers, metal chelate, Protein A,
epoxy)
Vivapure
ion exchange spin columns are available as ready-to-use kits and as
stand alone devices. The wide volume and capacity range meets every
need from initial screening to preparative protein purification.
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Product
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Application
|
Size
|
Volume
|
Binding
Capacity
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Chemistry
|
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Vivapure
IEX
Spin Columns
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Ion Exchange Chromatography
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Mini
Maxi
Mega
96 well
8-strip
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400/500 µl
19/20 ml
75 ml
800 µl
300 µl
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0.02 mg - 4.0 mg
15 mg - 80 mg
180 mg - 230 mg
200 µg/well
1 mg/well
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Q, S, C, D
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Vivapure
Protein A
Mini Spin Columns
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Affinity Chromatography
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Mini
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400 µl
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0.3 mg
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Protein A
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Vivapure
Metal Chelate
Mini Spin Columns
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Affinity Chromatography
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Mini
Maxi
Mega
96 well
8-strip
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400/500 µl
19/20 ml
75 ml
800 µl
300 µl
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400 µg
6 - 8 mg
18 - 20 mg
up to 30 µg/well
up to 350 µg/well
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Metal Chelate (IDA)
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Vivapure
Epoxy
Protein Coupling Kit
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Activated Membranes
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Mini
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100 µl - 400 µl
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0.2 mg - 0.5 mg
coupling capacity
per spin column
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Epoxy Groups
|
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Vivapure
Acidic
Protein Purification Kit
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Kits
|
Mini
Maxi
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400/500 µl
19/20 ml
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4 mg
60 mg - 80 mg
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IEX (Q)
|
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Vivapure
Basic
Protein Purification Kit
|
Kits
|
Mini
Maxi
|
400/500 µl
19/20 ml
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4 mg
60 mg - 80 mg
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IEX (S)
|
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DNA
Mini Devices
Vivapure DNA Removal Kit
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Kits
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Mini
Maxi
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400/500 µl
19/20 ml
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0.8 mg
4 mg
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IEX (D)
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Vivapure
C18
Micro spin columns
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Reversed Phase Chromatography
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Micro
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sample:
10-200 µl,
elution: 2-4 µl
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up to 5 µg
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C-18
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Vivapure
Anti-HSA Kit
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Human Albumin Depletion
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Mini
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Max. 300µl
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2mg/ml
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Anti-HSA antibody fragment
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Vivapure®
Seppro™
· Anti-BSA Kit
· MIXED 6 Kit
· MIXED 12 Kit
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abundant protein depletion
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Mini
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500 µl
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1 mg/ml
(see datasheets
for details)
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IgY
Anti-BSA
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Vivapure
Epoxy
Protein Coupling Kit
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Kits
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Mini
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100 µl - 400 µl
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0.2 mg - 0.5 mg
coupling capacity
per spin column
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Epoxy Groups
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Vivawell
8-strip IEX
centrifugal multiwell
purification strips
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HTS
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300 µl
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1 mg/well
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Q, S, D ,C
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Vivawell
8-strip
metal chelate
centrifugal strips
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HTS
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300 µl
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up to 350 µg/well
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Metal Chelate (IDA)
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Vivawell
96 IEX
multiwell purification plate
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HTS
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800 µl
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200 µl/well
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Q, S, D ,C
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Vivawell
96 metal chelate
centrifugal plate
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HTS
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800 µl
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up to 30 µg/well
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Metal Chelate (IDA)
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德国Sartorius下属Vivascience公司是专业蛋白质纯化分离技术研究及生产公司,不断有新产品推出以完善其产品体系。欲获得详细资料请浏览以下网站:
www.vivascience.com
www.biolab21.com
蛋白质分离和纯化的新武器
离子交换层析和离子交换色谱法是分离纯化蛋白最常用的方法,德国Sartorius利用以上技术巧妙设计出离子交换膜吸附柱(Vivapure)和离子交换膜正压滤器(Sartobind)用于分离纯化蛋白质,并克服了传统方法费时长、操作复杂、设备昂贵等不足。用德国Sartorius设计的产品分离和纯化蛋白速度快、操作简单、而且目标蛋白纯度大收率高。
典型应用:
☆
纯化和浓缩重组蛋白
☆
纯化和浓缩可溶性蛋白和膜蛋白
☆
纯化和浓缩从血清、腹水或组织培养液中获取的抗体
☆
蛋白质结构分析(如质谱分析、X-射线结构分析)前样品脱盐
☆
去除小分子杂质
☆
病毒的纯化和浓缩或去除病毒
☆
测定未知蛋白的等电点
☆
去除内毒素
☆
去除去垢剂
☆
去除糖
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所有产品可根据需要灵活选择离子交换膜:
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功能基团
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离子交换类型
|
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Sulphonic acid (S )
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强阳离子交换
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R- CH2- SO3- Na+
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Quaternary ammonoum (Q )
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强阴离子交换
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R-CH2- N+- (CH3)3Cl-
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Carboxylic acid (C )
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弱阳离子交换
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R-COO-
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Diethylamine ( D )
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弱阴离子交换
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R-CH2-N-(C2H5)2
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※ Vivapure->带离子交换膜的离心管
不到30分钟,即可得到高收率、高纯度蛋白质,膜的吸附容量从20ug-80mg
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品 名
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吸附容量
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蛋白质吸附量
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工作体积(ml)
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包装(套/kit)
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价格
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Vivapure Mini L
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低
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20 ug
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0.5**
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24
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760.00元/kit
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Vivapure Mini M
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中
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1 mg
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0.5**
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24
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1080.00元/kit
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Vivapure Mini H
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高
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4 mg
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0.4**
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24
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1780.00元/kit
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Vivapure Mini H 试用装(每种离子膜4个)
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16
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860.00元/kit
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Vivapure Maxi M
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中
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15-20 mg
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20*
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8
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1680.00元/kit
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Vivapure Maxi H
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高
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60-80 mg
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19*
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8
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3280.00元/kit
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*用水平转头离心时,每次最大处理量/
**用角转头离心,每次最大处理量
※ Sartobind带离子交换膜正压滤器
可快速得到高收率、高纯度蛋白质,处理速度每个滤器10ml-75ml/min,膜的吸附容量从2mg-200mg
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品 名
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膜材料
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吸附面积
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蛋白质吸附量
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处理速度
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包 装
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价 格
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MA5
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MBS-copolymer
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5cm2
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2-10mg
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>100ml/min
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15个/盒
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2800.00元/kit
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MA15
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polysulfone
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15cm2
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10-30mg
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>50ml/min
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2个/盒
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2600.00元/kit
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MA100
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polysulfone
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100cm2
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70-200mg
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>75ml/min
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1个/盒
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6900.00元/kit
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